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Rabbit Monoclonal SNAP25 antibody

目录号: PC-53038

Synaptosomal-Associated Protein, 25kDa (SNAP-25) is a Target Soluble NSF (N-ethylmaleimide-sensitive factor) Attachment Protein Receptor (t-SNARE) protein. SNAP-25 is a component of the trans-SNARE complex, which accounts for membrane fusion specificity and directly executes fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together. Molecular machinery driving exocytosis in neuromediator release. The core SNARE complex is formed by four α-helices contributed by synaptobrevin, syntaxin and SNAP-25. Synaptotagmin serves as a Ca2+ sensor and intimately regulates SNARE zipping. SNAP-25, a Q-SNARE protein, is anchored to the cytosolic face of membranes via palmitoyl side chains covalently bound to cysteine amino acid residues in the central linker domain of the molecule. 

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规格 价格 库存
50ul 1800.00 现货
100ul 3200.00 现货

基本信息

名称:兔单克隆 SNAP25 抗体

描述:Synaptosomal-Associated Protein, 25kDa (SNAP-25) is a Target Soluble NSF (N-ethylmaleimide-sensitive factor) Attachment Protein Receptor (t-SNARE) protein. SNAP-25 is a component of the trans-SNARE complex, which accounts for membrane fusion specificity and directly executes fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together. Molecular machinery driving exocytosis in neuromediator release. The core SNARE complex is formed by four α-helices contributed by synaptobrevin, syntaxin and SNAP-25. Synaptotagmin serves as a Ca2+ sensor and intimately regulates SNARE zipping. SNAP-25, a Q-SNARE protein, is anchored to the cytosolic face of membranes via palmitoyl side chains covalently bound to cysteine amino acid residues in the central linker domain of the molecule. 

克隆:单克隆

纯化方式:Protein A affinity purified.

宿主:Rabbit/IgG

浓度:1mg/ml

种属反应性:Human,Mouse,Rat

经测试应用:WB,IHC-P,IF/ICC

预测分子量:23kDa

阳性对照:SH-SY5Y cell lysate, Neuro-2a cell lysate, human brain tissue lysate, SH-SY5Y, human brain tissue, mouse brain tissue, rat brain tissue.

抗原:Recombinant protein within Human SNAP25 aa 51-150 / 206.

应用:  WB:1:500-2000        IHC-P:1:500-1000      ICC/IF:1:100   

存储形式:Liquid

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亚细胞定位:Cytoplasm, perinuclear region, Cell membrane; Lipid-anchor, Synapse, synaptosome, Photoreceptor inner segment.

存储液:1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

储存温度:-20℃

 

 

 

 

Western blot analysis of SNAP25 on different lysates with Rabbit anti-SNAP25 antibody at 1/2,000 dilution.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-SNAP25 antibody at 1/500 dilution.

 

 

 

 

 

 

 

 

 

 

 

 

 

Immunocytochemistry analysis of SH-SY5Y cells labeling SNAP25 with Rabbit anti-SNAP25 antibody at 1/100 dilution.

存储温度 -20℃

相关说明文档

储备液配置

注:请根据所选浓度进行配置储备液,配置好储备液后进行分装低温保存,避免反复冻融!PCM提供多种溶剂,如:DMSO(PC-57930);玉米油(PC-86094);棉籽油(PC-30875)等

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