产品背景：Interleukin-4 (IL-4) is a key cytokine produced by activated T cells, mast cells, basophils, neutrophils and eosinophils. IL-4 is critical for the development of Th2-mediated responses, which is related to allergy and asthma. It can also regulate B cell responses, including survival, cell proliferation and gene expression. IL-4 also plays fundamental role for B-cell stimulation, including induction of the IgE isotype switch.

蛋白编码：P07750

别名：interleukin 4, BCGF, BCDF, B-cell Stimulating Factor (BSF-1), IL4

分子量：The protein has a calculated MW of 14.5 kDa. The protein migrates as 14 kDa under reducing condition (SDS-PAGE analysis).

表达系统：Escherichia coli

纯度：>98% as determined by SDS-PAGE.

内毒素：< 0.1 EU/µg as determined by LAL test.

活性：Measure by its ability to induce HT-2 cells proliferation. The ED₅₀ for this effect is <1 ng/mL. The specific activity of recombinant mouse IL-4 is approximately >1 x 10⁶ IU/mg.

序列：MHIHGCDKNHLREIIGILNEVTGEGTPCTEMDVPNVLTATKNTTESELVCRASKVLRIFYLKHGKTPCLKKNSSVLMELQRLFRAFRCLDSSISCTMNESKSTSLKDFLESLKSIMQMDYS with polyhistidine tag at the C-terminus.

标签：His Tag (C-term)

产品形式：The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4.

SDS-PAGE analysis of recombinant mouse IL-4

干粉复溶：

Always centrifuge tubes before opening.Do not mix by vortex or pipetting.

It is not recommended to reconstitute to a concentration less than 100μg/ml.

Dissolve the lyophilized protein in distilled water.

Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

保存条件：Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.

Reconstituted protein solution can be stored at 2-8°C for 2-7 days.

Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.