名称：小鼠单克隆CD31抗体

描述：4-hydroxy-2-nonenal (4-hydroxynonenal, 4-HNE) is a highly reactive aldehyde generated by the exposure of polyunsaturated fatty acids to peroxides and reactive oxygen species (ROS). It non-enzymatically forms stable protein adducts with histidine, lysine, and cysteine side chains that have been used as biomarkers for oxidative damage in cells. Conditions where 4-HNE immunoreactivity has been observed include include inflammation, neurodegenerative diseases, and ischemic damage to the heart and brain.

特异性：Detects 4-Hydroxynonenal adducts of histidine residues. No cross-reactivity with nitrotyrosine, formaldehyde, glutaraldehyde, or 4‑hydroxy hexenaldehyde adducts is observed.

克隆：单克隆 

同种型：IgG_2B

宿主：Mouse

种属反应性：Human，Mouse，Sheep

经测试应用：WB，IF/ICC，IHC-P，IHC-Fr

形式：Liquid

浓度：WB：1:100-500     IF/ICC：1:50-300    IHC-P/IHC-Fr：1:50-300  

存储液：PBS, pH 7.4

储存温度：长时间-20℃,短时间内储存于2-8℃，避免污染

使用小鼠单克隆4-HNE抗体检测人HepG2细胞的裂解液未经处理（-）或经4-羟基壬烯醛（4-HNE）处理（+）后，Western blot结果显示，检测到4-HNE 与蛋白中组氨酸（histidine）残基形成的加合物（adducts）的特异性信号（如图所示）。

ECL：PC-30002

使用小鼠单克隆4-HNE抗体在石蜡包埋人前列腺癌组织切片中检测，稀释比1:100，在孵育一抗之前需进行抗原修复。