产品背景：Granulocyte-macrophage colony-stimulating factor (GM-CSF) was first identified as a Growth Factors due to its ability to induce proliferation and differentiation of bone marrow progenitors into granulocytes and macrophages. GM-CSF is produced by multiple cell types including activated T cells, B cells, macrophages, endothelial cells and fibroblasts upon receiving immune stimuli. GM-CSF stimulates stem cells to produce granulocytes and monocytes functions as a cytokine.

蛋白编码：P01587

别名：granulocyte-macrophage colony-stimulating factor, colony stimulating factor 2, CSF2, CSF-2

分子量：The protein has a calculated MW of 15.1 kDa. The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).

表达系统：Escherichia coli

纯度：>98% as determined by SDS-PAGE and SEC-HPLC.

内毒素：< 0.1 EU/µg as determined by LAL test.

活性：Measure by its ability to induce proliferation in FDC-P1 cells. The ED₅₀ for this effect is <50 pg/mL. The specific activity of recombinant mouse GM-CSF is approximately >2 x 10⁷ IU/mg.

序列：APTRSPITVTRPWKHVEAIKEALNLLDDMPVTLNEEVEVVSNEFSFKKLTCVQTRLKIFEQGLRGNFTKLKGALNMTASYYQTYCPPTPETDCETQVTTYADFIDSLKTFLTDIPFECKKPVQK with polyhistidine tag at the N-terminus.

标签：His Tag (N-term)

产品形式：The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4.

SDS-PAGE analysis of recombinant mouse GM-CSF

干粉复溶：

Always centrifuge tubes before opening.Do not mix by vortex or pipetting.

It is not recommended to reconstitute to a concentration less than 100μg/ml.

Dissolve the lyophilized protein in distilled water.

Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

保存条件：Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.

Reconstituted protein solution can be stored at 2-8°C for 2-7 days.

Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.