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Rabbit Monoclonal alpha smooth muscle Actin Antibody

目录号: PC-78052

All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β-Actin and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.

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规格 价格 库存
50ul 1800.00 现货
100ul 3200.00 现货

基本信息

名称:兔单克隆α-SMA抗体

描述:All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β-Actin and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.

克隆:单克隆

浓度: 1mg/ml

同种型:IgG

宿主:Rabbit

种属反应性:Human,Mouse,Rat

经测试应用:WB,IF/ICC,IHC-P,IHC-Fr,FC

预测分子量:42kDa

抗原:Synthetic peptide within N-terminal human alpha smooth muscle Actin.

阳性对照:Saos-2 cell lysate, A431 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, Neuro-2a cell lysate, mouse skin tissue lysate, rat skin tissue lysate, human breast carcinoma tissue, HeLa cell lysate, A431 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, L6 cell lysate, mouse heart tissue lysate, mouse skin tissue lysate, rat heart tissue lysate, rat skin tissue lysate, rat smooth muscle tissue lysate, human kidney tissue, human stomach cancer tissue, human pancreatic carcinoma, HepG2, human tonsil tissue, human liver tissue, human gastric cancer, Human non-small cell lung cancer, mouse pancreas, mouse liver, mouse kidney, human stomach cancer tissue, human small intestine tissue, mouse small intestine tissue, rat small intestine tissue, NIH/3T3, PC-12, HepG2.

形式:Liquid

浓度:WB:1:1000-3000  IF/ICC:1:200-800   IHC-P:1:1000-2000  FC:1:500-1000

存储液:1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

储存温度:-20℃,避免污染

Western blot analysis of alpha smooth muscle Actin on different lysates with Rabbit anti-alpha smooth muscle Actin antibody (1:5000)

对石蜡包埋的人肾组织使用兔单克隆α-SMA抗体(1:800)进行免疫荧光分析

使用α-SMA抗体对NIH/3T3细胞进行流式细胞分析,红色为标记后的α-SMA,绿色为兔IgG同行对照,黑色为未结合α-SMA

 

 

存储温度 -20℃

相关说明文档

储备液配置

注:请根据所选浓度进行配置储备液,配置好储备液后进行分装低温保存,避免反复冻融!PCM提供多种溶剂,如:DMSO(PC-57930);玉米油(PC-86094);棉籽油(PC-30875)等

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